Protocol for Transplantation of Healthy Cells Between Adult Drosophila of Different Ages and Sexes

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Published: 2025-02-19
DOI: https://doi.org/10.5281/zenodo.14889948
Keywords:
аllotransplantation, drosophila melanogaster,, sexual dimorphism, pathway, aging, signaling

Main Article Content

Jaba Tkemaladze
Longevity Clinic
https://orcid.org/0000-0001-8651-7243

Abstract

Drosophila melanogaster serves as a powerful and versatile model organism for studying tissue allotransplantation due to its short life cycle, genetic manipulability, and significant homology to mammalian signaling pathways. This protocol outlines a procedure for performing tissue transplants between adult individuals of different ages and sexes. Key steps include dissection of the donor’s midgut tissue, microinjection into the recipient, and tracking engraftment using sex chromosome differences. The protocol demonstrates high short-term survival (over 80%) of host organisms, with transplanted tissues encapsulated by hemocytes. Sexual dimorphism affects transplant outcomes, with females showing stronger immune responses through the Toll pathway, resulting in more frequent rejections, while males exhibit greater tissue tolerance. Age-related factors, including reduced regenerative capacity and oxidative stress in older individuals, impact transplantation success. This methodology also enables modeling of intestinal stem cell regeneration. Despite challenges such as small tissue size and lack of an adaptive immune system, the protocol offers valuable insights into innate immunity, aging, and intercellular interactions, positioning Drosophila as an ideal preclinical model for studying tissue regeneration and immune response.

Article Details

Issue

Vol. 1 No. 2 (2025)

Section

Technology and Innovations
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Articles published in Longevity Horizon fall under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). This means authors retain their copyright while granting the journal the right to be the first publisher. Readers are permitted to share and distribute the work as long as proper credit is given. However, the content may not be altered, modified, or used for commercial purposes.

Author Biography

Jaba Tkemaladze, Longevity Clinic

Dr Jaba Tkemaladze is a Professor, a Scientist, and a President of Longevity Alliance Georgia.

Research Director at Longevity Clinic.

Replacing old adult stem cells with induced and safe young adult stem cells.

World-renowned scientist. Developed the Centriolar theory of differentiation and the Centriolar theory of organism ageing. With acquired experience in both academia and industry.

Training in medicine at Tbilisi State Medical University and then at the Psychiatry Research Institute further deepened my knowledge in the laboratory of the Institute of Morphology. Namely, combined experimental and computational methods to study the ageing process and the various ways of manipulating age-related diseases and improvement of human health.

Also served as a Scientific Advisory Board Member in Georgia's Ministry of Defense and Longevity Alliance. Published over 50 scientific articles, given over 100 invited talks and received several awards.

His Rejuvenation Formula: Rejuvenation = Replacement of Old Centrioles with Young Ones.

How to Cite

Tkemaladze, J. (2025). Protocol for Transplantation of Healthy Cells Between Adult Drosophila of Different Ages and Sexes. Longevity Horizon, 1(2). DOI:https://doi.org/10.5281/zenodo.14889948
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