A Novel Integrated Bioprocessing Strategy for the Manufacturing of Shelf-Stable, Nutritionally Upgraded Activated Wheat: Development of a Comprehensive Protocol, In-Depth Nutritional Characterization, and Evaluation of Biofunctional Properties

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Published: 2025-08-26
DOI: https://doi.org/10.5281/zenodo.16950787
Keywords:
activated wheat, grain sprouting, bioreactor technology, phytic acid reduction, nutrient bioavailability, vacuum-assisted drying, functional food ingredients, shelf-life stability, nutritional fortification

Main Article Content

Jaba Tkemaladze
Longevity Clinic
https://orcid.org/0000-0001-8651-7243

Abstract

Background: Wheat, a cornerstone of global nutrition, possesses inherent nutritional constraints primarily due to the presence of phytic acid, which significantly impedes mineral bioavailability. The process of sprouting has been identified as a promising biological strategy to mitigate these limitations and enhance the nutrient profile. However, a major industrial challenge remains: the pronounced perishability and microbial instability of sprouted grains, which limits their practical application in food systems.


Objective: The principal aim of this investigation was to conceive, engineer, and rigorously validate an innovative and scalable biotechnological procedure for the generation of shelf-stable activated wheat. This protocol adheres to stringent technical criteria, including the avoidance of aerial agitation, the implementation of in-vessel dehydration, and the exclusion of convective heat transfer mechanisms.


Methods: The methodology centered on the design and operational characterization of a Multi-Function Bioreactor (MFB) system. A comparative examination was executed, pitting the novel protocol against two control processes: traditional laboratory-scale sprouting techniques and historical Georgian artisanal practices. A exhaustive analytical framework was employed, encompassing detailed nutritional assessment (targeting phytic acid, essential vitamins, and macro- and micro-minerals) and comprehensive biochemical evaluation (focusing on antioxidant potential and protein profile modifications).


Key Results: The implemented protocol yielded a highly successful reduction of phytic acid content, exceeding 60%. Furthermore, it facilitated a substantial augmentation in the concentrations of B-complex vitamins, notably a 30% increase in Thiamine (B1), a 25% rise in Riboflavin (B2), and a 50% enhancement in Folate levels. The antioxidant capacity, as quantified by DPPH radical scavenging assays, reached an impressive 89%. Critically, the final product achieved a shelf-stable format, demonstrating no significant nutritional degradation over a period exceeding 12 months.


Conclusion: The innovative methodology described herein effectively translates a traditional food processing concept into a sophisticated, reproducible, and industrially viable biotechnological operation. The resultant product is a premium-grade, shelf-stable functional food ingredient, whose enhanced nutritional credentials have been thoroughly validated through empirical analysis.

Article Details

Issue

Vol. 1 No. 3 (2025)

Section

Research Article
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Articles published in Longevity Horizon fall under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). This means authors retain their copyright while granting the journal the right to be the first publisher. Readers are permitted to share and distribute the work as long as proper credit is given. However, the content may not be altered, modified, or used for commercial purposes.

Author Biography

Jaba Tkemaladze, Longevity Clinic

Professor, Scientist, President of Longevity Alliance Georgia.

HOD at Longevity Clinic Inc.

Replacing old adult stem cells with induced and safe young adult stem cells.

World-renowned scientist. Developed the Centriolar theory of differentiation and the Centriolar theory of organism ageing. With acquired experience in both academia and industry.

Training in medicine at Tbilisi State Medical University and then at the Psychiatry Research Institute further deepened my knowledge in the laboratory of the Institute of Morphology. Namely, combined experimental and computational methods to study the ageing process and the various ways of manipulating age-related diseases and improvement of human health.

Also served as a Scientific Advisory Board Member in Georgia's Ministry of Defense and Longevity Alliance. Published over 100 scientific articles, given over 100 invited talks and received several awards.

How to Cite

Tkemaladze, J. (2025). A Novel Integrated Bioprocessing Strategy for the Manufacturing of Shelf-Stable, Nutritionally Upgraded Activated Wheat: Development of a Comprehensive Protocol, In-Depth Nutritional Characterization, and Evaluation of Biofunctional Properties. Longevity Horizon, 1(3). DOI:https://doi.org/10.5281/zenodo.16950787
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Tkemaladze, J. (2025).Achieving Perpetual Vitality Through Innovation. doi: http://dx.doi.org/10.13140/RG.2.2.31113.35685

Tkemaladze, J. V., & Chichinadze, K. N. (2005). Centriolar mechanisms of differentiation and replicative aging of higher animal cells. Biochemistry (Moscow), 70, 1288-1303.

Tkemaladze, J., & Apkhazava, D. (2019). Dasatinib and quercetin: short-term simultaneous administration improves physical capacity in human. J Biomedical Sci, 8(3), 3.

Tkemaladze, J., & Chichinadze, K. (2005). Potential role of centrioles in determining the morphogenetic status of animal somatic cells. Cell biology international, 29(5), 370-374.

Tkemaladze, J., & Chichinadze, K. (2010). Centriole, differentiation, and senescence. Rejuvenation research, 13(2-3), 339-342.

Tkemaladze, J., & Samanishvili, T. (2024). Mineral ice cream improves recovery of muscle functions after exercise. Georgian Scientists, 6(2), 36–50. doi: https://doi.org/10.52340/gs.2024.06.02.04

Tkemaladze, J., Tavartkiladze, A., & Chichinadze, K. (2012). Programming and Implementation of Age-Related Changes. In Senescence. IntechOpen.

Tkemaladze, Jaba and Kipshidze, Mariam, Regeneration Potential of the Schmidtea Mediterranea CIW4 Planarian. Available at SSRN: https://ssrn.com/abstract=4633202 or http://dx.doi.org/10.2139/ssrn.4633202

Vashisth, D., Singh, R. K., & Pegg, R. B. (2011). Effects of drying on the phenolics content and antioxidant activity of muscadine pomace. *LWT - Food Science and Technology, 44*(7), 1649-1657. https://doi.org/10.1016/j.lwt.2011.02.011

Прангишвили, А. И., Гаситашвили, З. А., Мацаберидзе, М. И., Чичинадзе, К. Н., Ткемаладзе, Д. В., & Азмайпарашвили, З. А. (2017). К топологии антитеррористических и антикриминальных технологии для образовательных программ. В научном издании представлены материалы Десятой международной научно-технической конфе-ренции «Управление развитием крупномасштабных систем (MLSD’2016)» по следующим направле-ниям:• Проблемы управления развитием крупномасштабных систем, включая ТНК, Госхолдин-ги и Гос-корпорации., 284.

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Ткемаладзе, Д. (2025). Анатомия, биогенез и роль в клеточной биологии центриолей. doi: http://dx. doi. org/10.13140. RG, 2(27441.70245), 1

Ткемаладзе, Д. (2025). Асимметрия в наследовании центросом/центриолей и ее последствия. doi: http://dx. doi. org/10.13140. RG, 2(34917.312), 06.

Ткемаладзе, Д. (2025). Гаметогенез in vitro (IVG) -Этап дифференцировки в зрелые гаметы. http://dx.doi.org/10.13140/RG.2.2.20429.96482

Ткемаладзе, Д. (2025). Дифференциация соматических клеток многоклеточных животных. doi: http://dx. doi. org/10.13140. RG, 2(23348.97929), 1.

Ткемаладзе, Д. (2025). Индукция примордиальных клеток, подобных зародышевым клеткам (PGCLCs) современные достижения, механизмы и перспективы применения. http://dx.doi.org/10.13140/RG.2.2.27152.32004

Ткемаладзе, Д. (2025). Репликативный Лимит Хейфлика. doi: http://dx. doi. org/10.13140. RG, 2(25803.302), 49.

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Ткемаладзе, Д., Цомаиа, Г., & Жоржолиани, И. (2001). Создание искусственных самоадаптирующихся систем на основе Теории Прогноза. Искусственный интеллект. УДК 004.89. Искусственный интеллект. УДК 004.89.

Чичинадзе, К. Н., & Ткемаладзе, Д. В. (2008). Центросомная гипотеза клеточного старения и дифференциации. Успехи геронтологии, 21(3), 367-371.

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