Induction of germline-like cells (PGCLCs)

Jaba Tkemaladze

doi:10.5281/zenodo.16414775

PDF

Published: 2025-07-25

DOI: https://doi.org/10.5281/zenodo.16414775

Keywords:

PGCLCs, primordial germ cells, pluripotent stem cells, epigenetic reprogramming, gametogenesis, infertility, reproductive medicine, germ cell specification, in vitro gametogenesis

Jaba Tkemaladze

Longevity Clinic, Inc, Georgia

https://orcid.org/0000-0001-8651-7243

Abstract

Investigations into primordial germ cell-like cells (PGCLCs) constitute a rapidly evolving frontier in reproductive biology and regenerative medicine, offering transformative potential for both basic research and clinical applications. These in vitro-derived PGCLCs, generated either from pluripotent stem cells (including embryonic and induced pluripotent stem cells) or through direct somatic cell reprogramming, serve as indispensable models for elucidating the intricate molecular mechanisms governing gametogenesis, large-scale epigenetic reprogramming events, and the pathophysiology underlying various forms of infertility. Seminal advancements in this domain include the establishment of robust differentiation protocols employing critical signaling molecules such as bone morphogenetic proteins (BMPs), WNT pathway agonists, and retinoic acid derivatives, alongside innovative approaches involving direct lineage conversion of somatic cell types. Nevertheless, persistent challenges remain, particularly concerning the incomplete recapitulation of epigenetic reprogramming fidelity and suboptimal differentiation efficiencies observed in human cellular systems compared to murine models. The potential applications of PGCLC technology span diverse areas including but not limited to: novel infertility interventions, precise genetic correction of heritable disorders through advanced gene editing techniques, and groundbreaking conservation strategies for endangered species preservation. Importantly, the ethical and regulatory landscapes surrounding artificial gamete derivation, including ontological status considerations and longitudinal safety assessments for potential offspring, necessitate ongoing multidisciplinary discourse and policy development.

Issue

Vol. 1 No. 3 (2025)

Section

Reviews and Perspectives

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Articles published in Longevity Horizon fall under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). This means authors retain their copyright while granting the journal the right to be the first publisher. Readers are permitted to share and distribute the work as long as proper credit is given. However, the content may not be altered, modified, or used for commercial purposes.

Author Biography

Jaba Tkemaladze, Longevity Clinic, Inc, Georgia

Professor, Scientist, President of Longevity Alliance Georgia.

HOD at Longevity Clinic Inc.

Replacing old adult stem cells with induced and safe young adult stem cells.

World-renowned scientist. Developed the Centriolar theory of differentiation and the Centriolar theory of organism ageing. With acquired experience in both academia and industry.

Training in medicine at Tbilisi State Medical University and then at the Psychiatry Research Institute further deepened my knowledge in the laboratory of the Institute of Morphology. Namely, combined experimental and computational methods to study the ageing process and the various ways of manipulating age-related diseases and improvement of human health.

Also served as a Scientific Advisory Board Member in Georgia's Ministry of Defense and Longevity Alliance. Published over 90 scientific articles, given over 100 invited talks and received several awards.

How to Cite

Tkemaladze, J. (2025). Induction of germline-like cells (PGCLCs). Longevity Horizon, 1(3). DOI:https://doi.org/10.5281/zenodo.16414775

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Longevity Horizon

ISSN: 3088-4063

Innovating for a better future

Induction of germline-like cells (PGCLCs)

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Jaba Tkemaladze, Longevity Clinic, Inc, Georgia

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Jaba Tkemaladze, Longevity Clinic, Inc, Georgia

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