Mesenchymal Progenitor Cell Resilience Counteracts Tissue Aging
DOI:
https://doi.org/10.65649/c833dn40Keywords:
Cellular Senescence, Mesenchymal Stromal Cells, Aging, Osteoporosis, Regenerative Medicine, Stem Cell Heterogeneity, DNA Repair, EpigeneticsAbstract
The age-related functional decline of mesenchymal stromal/progenitor cells (MSCs) is a central driver of skeletal aging, leading to osteoporosis and impaired tissue regeneration (Li, Wu, & Tuan, 2019). Challenging the paradigm of a uniformly aging MSC pool, this study identifies a rare subpopulation of mesenchymal progenitors with innate senescence resistance, termed Senescence-Resistant Committed progenitors (SRCs). Isolated from aged murine and human tissues via a unique immunophenotype (CD45⁻CD31⁻CD34⁻CD73⁺CD90⁺CD105⁺CD200⁺ITGA10⁺), SRCs demonstrated remarkable numerical preservation compared to severely depleted classical MSCs. Functionally, aged SRCs maintained low senescence markers (p16INK4a, SA-β-gal), lacked a pro-inflammatory secretome, and retained high clonogenic, proliferative, and osteogenic potential, countering the age-related adipogenic shift (Moerman, Teng, Lipschitz, & Lecka-Czernik, 2004). Transcriptomic and epigenetic profiling revealed that SRC resilience was underpinned by constitutive activation of DNA repair, antioxidant defense (NRF2), and mitochondrial homeostasis pathways, stabilized by a protective epigenetic landscape featuring *CDKN2A/p16* promoter hypomethylation (Sun, Coppe, & Lam, 2018). In vivo, transplantation of aged SRCs significantly enhanced bone regeneration in critical-sized defects compared to aged bulk MSCs. This discovery reframes mesenchymal aging, revealing a resilient cellular reservoir essential for tissue homeostasis. Targeting the SRC pool or mimicking its molecular signature represents a novel therapeutic frontier for age-related skeletal disorders.
References
Ambrosi, T. H., Scialdone, A., Graja, A., Gohlke, S., Jank, A. M., Bocian, C., & Schulz, T. J. (2017). Adipocyte accumulation in the bone marrow during obesity and aging impairs stem cell-based hematopoietic and bone regeneration. Cell Stem Cell, 20(6), 771-784.e6. https://doi.org/10.1016/j.stem.2017.02.009
Aphkhazava, D., Sulashvili, N., & Tkemaladze, J. (2025). Stem Cell Systems and Regeneration. Georgian Scientists, 7(1), 271–319. DOI : https://doi.org/10.52340/gs.2025.07.01.26
Aphkhazava, D., Sulashvili, N., Maglakelidze, G., & Tkemaladze, J. (2025). Ageless Creatures: Molecular Insights into Organisms That Defy Aging. Georgian Scientists, 7(3), 346–396. DOI : https://doi.org/10.52340/gs.2025.07.03.24
Baker, D. J., Wijshake, T., Tchkonia, T., LeBrasseur, N. K., Childs, B. G., van de Sluis, B., & van Deursen, J. M. (2011). Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders. Nature, 479(7372), 232–236. https://doi.org/10.1038/nature10600
Baryawno, N., Przybylski, D., Kowalczyk, M. S., Kroury, Y., Severe, N., Gustafsson, K., & Scadden, D. T. (2019). A cellular taxonomy of the bone marrow stroma in homeostasis and leukemia. Cell, 177(7), 1915-1932.e16. https://doi.org/10.1016/j.cell.2019.04.040
Buenrostro, J. D., Giresi, P. G., Zaba, L. C., Chang, H. Y., & Greenleaf, W. J. (2013). Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nature Methods, 10(12), 1213–1218. https://doi.org/10.1038/nmeth.2688
Chichinadze, K. N., & Tkemaladze, D. V. (2008). Centrosomal hypothesis of cellular aging and differentiation. Advances in Gerontology= Uspekhi Gerontologii, 21(3), 367-371.
Chichinadze, K., Lazarashvili, A., & Tkemaladze, J. (2013). RNA in centrosomes: structure and possible functions. Protoplasma, 250(1), 397-405.
Chichinadze, K., Tkemaladze, D., & Lazarashvili, A. (2012). New class of RNA and centrosomal hypothesis of cell aging. Advances in Gerontology= Uspekhi Gerontologii, 25(1), 23-28.
Chichinadze, K., Tkemaladze, J., & Lazarashvili, A. (2012). A new class of RNAs and the centrosomal hypothesis of cell aging. Advances in Gerontology, 2(4), 287-291.
Chichinadze, K., Tkemaladze, J., & Lazarashvili, A. (2012). Discovery of centrosomal RNA and centrosomal hypothesis of cellular ageing and differentiation. Nucleosides, Nucleotides and Nucleic Acids, 31(3), 172-183.
Childs, B. G., Durik, M., Baker, D. J., & van Deursen, J. M. (2015). Cellular senescence in aging and age-related disease: from mechanisms to therapy. Nature Medicine, 21(12), 1424–1435. https://doi.org/10.1038/nm.4000
Crisan, M., Yap, S., Casteilla, L., Chen, C. W., Corselli, M., Park, T. S., & Peault, B. (2008). A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell, 3(3), 301–313. https://doi.org/10.1016/j.stem.2008.07.003
Elfettahi, A. E., & Tkemaladze, J.(2025). The Neuro-Hepatic-Affective Model (NHAM): A Systems Framework for Liver–Brain Modulation of Emotion in Precision Psychiatry. Preprints. DOI : https://doi. org/10.20944/preprints202508, 1312, v1.
Fuhrmann-Stroissnigg, H., Ling, Y. Y., Zhao, J., McGowan, S. J., Zhu, Y., Brooks, R. W., & Niedernhofer, L. J. (2017). Identification of HSP90 inhibitors as a novel class of senolytics. Nature Communications, 8(1), 422. https://doi.org/10.1038/s41467-017-00314-z
Gattazzo, F., Urciuolo, A., & Bonaldo, P. (2014). Extracellular matrix: a dynamic microenvironment for stem cell niche. *Biochimica et Biophysica Acta (BBA) - General Subjects, 1840*(8), 2506–2519. https://doi.org/10.1016/j.bbagen.2014.01.010
Gorgoulis, V., Adams, P. D., & Alimonti, A. (2019). Cellular Senescence: Defining a Path Forward. Cell, 179(4), 813–827. https://doi.org/10.1016/j.cell.2019.10.005
Jaba, T. (2022). Dasatinib and quercetin: short-term simultaneous administration yields senolytic effect in humans. Issues and Developments in Medicine and Medical Research Vol. 2, 22-31.
Justesen, J., Stenderup, K., Kassem, M., & Mosekilde, L. (2002). Reciprocal changes in osteoblast and adipocyte differentiation during ageing: a role for estrogen? Journal of Bone and Mineral Research, 17(S1), S141.
Kipshidze, M., & Tkemaladze, J. (2023). Comparative Analysis of drugs that improve the Quality of Life and Life Expectancy. Junior Researchers, 1(1), 184–193. DOI : https://doi.org/10.52340/2023.01.01.19
Kipshidze, M., & Tkemaladze, J. (2023). The planaria Schmidtea mediterranea as a model system for the study of stem cell biology. Junior Researchers, 1(1), 194–218. DOI : https://doi.org/10.52340/2023.01.01.20
Kipshidze, M., & Tkemaladze, J. (2024). Abastumani Resort: Balneological Heritage and Modern Potential. Junior Researchers, 2(2), 126–134. DOI : https://doi.org/10.52340/jr.2024.02.02.12
Kipshidze, M., & Tkemaladze, J. (2024). Balneology in Georgia: traditions and modern situation. Junior Researchers, 2(2), 78–97. DOI : https://doi.org/10.52340/jr.2024.02.02.09
Kipshidze, M., & Tkemaladze, J. (2024). Microelementoses-history and current status. Junior Researchers, 2(2), 108–125. DOI : https://doi.org/10.52340/jr.2024.02.02.11
Lezhava, T., Monaselidze, J., Jokhadze, T., Kakauridze, N., Khodeli, N., Rogava, M., Tkemaladze, J., ... & Gaiozishvili, M. (2011). Gerontology research in Georgia. Biogerontology, 12, 87-91. DOI : 10.1007/s10522-010-9283-6. Epub 2010 May 18. PMID: 20480236; PMCID: PMC3063552
Li, Y., Wu, Q., & Tuan, R. S. (2019). Ageing of mesenchymal stem cells: Implications for regenerative medicine. Journal of Cellular and Molecular Medicine, 23(1), 8–16. https://doi.org/10.1111/jcmm.13969
López-Otín, C., Blasco, M. A., Partridge, L., Serrano, M., & Kroemer, G. (2013). The hallmarks of aging. Cell, 153(6), 1194–1217. https://doi.org/10.1016/j.cell.2013.05.039
Matsaberidze, M., Prangishvili, A., Gasitashvili, Z., Chichinadze, K., & Tkemaladze, J. (2017). TO TOPOLOGY OF ANTI-TERRORIST AND ANTI-CRIMINAL TECHNOLOGY FOR EDUCATIONAL PROGRAMS. International Journal of Terrorism & Political Hot Spots, 12.
Moerman, E. J., Teng, K., Lipschitz, D. A., & Lecka-Czernik, B. (2004). Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells: the role of PPAR-γ2 transcription factor and TGF-β/BMP signaling pathways. Aging Cell, 3(6), 379–389. https://doi.org/10.1111/j.1474-9728.2004.00127.x
Morita, Y., Ema, H., & Nakauchi, H. (2010). Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment. Journal of Experimental Medicine, 207(6), 1173–1182. https://doi.org/10.1084/jem.20091318
Pinho, S., Lacombe, J., Hanoun, M., Mizoguchi, T., Bruns, I., Kunisaki, Y., & Frenette, P. S. (2013). PDGFRα and CD51 mark human nestin+ sphere-forming mesenchymal stem cells capable of hematopoietic progenitor cell expansion. Journal of Experimental Medicine, 210(7), 1351–1367. https://doi.org/10.1084/jem.20122252
Prangishvili, A., Gasitashvili, Z., Matsaberidze, M., Chkhartishvili, L., Chichinadze, K., Tkemaladze, J., ... & Azmaiparashvili, Z. (2019). SYSTEM COMPONENTS OF HEALTH AND INNOVATION FOR THE ORGANIZATION OF NANO-BIOMEDIC ECOSYSTEM TECHNOLOGICAL PLATFORM. Current Politics and Economics of Russia, Eastern and Central Europe, 34(2/3), 299-305.
Schultz, M. B., & Sinclair, D. A. (2016). When stem cells grow old: phenotypes and mechanisms of stem cell aging. Development, 143(1), 3–14. https://doi.org/10.1242/dev.130633
Science, 1(1). DOI : https://doi.org/10.65649/yx9sn772
Stenderup, K., Justesen, J., Clausen, C., & Kassem, M. (2003). Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. Bone, 33(6), 919–926. https://doi.org/10.1016/j.bone.2003.07.005
Sun, Y., Coppe, J. P., & Lam, E. W. F. (2018). Cellular senescence: The sought or the unwanted? Trends in Molecular Medicine, 24(10), 871–885. https://doi.org/10.1016/j.molmed.2018.08.002
Tikhonova, A. N., Dolgalev, I., Hu, H., Sivaraj, K. K., Hoxha, E., Cuesta-Dominguez, Á., & Aifantis, I. (2019). The bone marrow microenvironment at single-cell resolution. Nature, 569(7755), 222–228. https://doi.org/10.1038/s41586-019-1104-8
Tkemaladze, J. (2025). Bayesian Principles in Ze Systems. Preprints. DOI : https://doi.org/10.20944/preprints202510.1287.v1
Tkemaladze, J. (2025). Concept of Death Awareness as an Existential Alarm Clock in the Context of Hypothetical Biological Immortality. Preprints. DOI : https://doi.org/10.20944/preprints202510.1067.v1
Tkemaladze, J. (2025). Lakes as Strategic Food Reserves. Preprints. DOI : https://doi.org/10.20944/preprints202510.2035.v1
Tkemaladze, J. (2025). Rejuvenation Biotechnology as a Civilizational Safeguard. Preprints. DOI : https://doi.org/10.20944/preprints202511.1795.v1
Tkemaladze, J. (2025). The Heroic Self-Myth Hypothesis: A Neuro-Phenomenological Framework for Pathological Self-Narrativization in the Modernist Epoch. Preprints. DOI : https://doi.org/10.20944/preprints202511.1774.v1
Tkemaladze, J. (2025). The Tkemaladze Method: Mapping Cell Lineage with Mutant Mitochondrial Transfer. Preprints. DOI : https://doi.org/10.20944/preprints202509.2586.v1
Tkemaladze, J. (2025). The Weak Gilgamesh and the Strong Enkidu. Preprints. DOI : https://doi.org/10.20944/preprints202512.1216.v1
Tkemaladze, J. (2025). Uznadze’s Theory of Set: Experimental Diagnostics and Neurocognitive Implications. Preprints. DOI : https://doi.org/10.20944/preprints202511.1006.v1
Tkemaladze, J. (2025). Voynich Manuscript Decryption: A Novel Compression-Based Hypothesis and Computational Framework. Preprints. https://doi.org/10.20944/preprints202509.0403.v1
Tkemaladze, J. (2025). Ze-HB Hierarchical Bayesian Extension of the Ze. Preprints. DOI : https://doi.org/10.20944/preprints202512.0103.v1
Tkemaladze, J. (2023). Cross-senolytic effects of dasatinib and quercetin in humans. Georgian Scientists, 5(3), 138–152. DOI : https://doi.org/10.52340/2023.05.03.15
Tkemaladze, J. (2023). Is the selective accumulation of oldest centrioles in stem cells the main cause of organism ageing?. Georgian Scientists, 5(3), 216–235. DOI : https://doi.org/10.52340/2023.05.03.22
Tkemaladze, J. (2023). Long-Term Differences between Regenerations of Head and Tail Fragments in Schmidtea Mediterranea Ciw4. Available at SSRN 4257823.
Tkemaladze, J. (2023). Reduction, proliferation, and differentiation defects of stem cells over time: a consequence of selective accumulation of old centrioles in the stem cells?. Molecular Biology Reports, 50(3), 2751-2761. DOI : https://pubmed.ncbi.nlm.nih.gov/36583780/
Tkemaladze, J. (2023). Structure and possible functions of centriolar RNA with reference to the centriolar hypothesis of differentiation and replicative senescence. Junior Researchers, 1(1), 156–170. DOI : https://doi.org/10.52340/2023.01.01.17
Tkemaladze, J. (2023). The centriolar hypothesis of differentiation and replicative senescence. Junior Researchers, 1(1), 123–141. DOI : https://doi.org/10.52340/2023.01.01.15
Tkemaladze, J. (2024). Absence of centrioles and regenerative potential of planaria. Georgian Scientists, 6(4), 59–75. DOI : https://doi.org/10.52340/gs.2024.06.04.08
Tkemaladze, J. (2024). Cell center and the problem of accumulation of oldest centrioles in stem cells. Georgian Scientists, 6(2), 304–322. DOI : https://doi.org/10.52340/gs.2024.06.02.32
Tkemaladze, J. (2024). Editorial: Molecular mechanism of ageing and therapeutic advances through targeting glycative and oxidative stress. Front Pharmacol. 2024 Mar 6;14:1324446. DOI : 10.3389/fphar.2023.1324446. PMID: 38510429; PMCID: PMC10953819.
Tkemaladze, J. (2024). Elimination of centrioles. Georgian Scientists, 6(4), 291–307. DOI : https://doi.org/10.52340/gs.2024.06.04.25
Tkemaladze, J. (2024). Main causes of intelligence decrease and prospects for treatment. Georgian Scientists, 6(2), 425–432. DOI : https://doi.org/10.52340/gs.2024.06.02.44
Tkemaladze, J. (2024). The rate of stem cell division decreases with age. Georgian Scientists, 6(4), 228–242. DOI : https://doi.org/10.52340/gs.2024.06.04.21
Tkemaladze, J. (2025). A Universal Approach to Curing All Diseases: From Theoretical Foundations to the Prospects of Applying Modern Biotechnologies in Future Medicine. DOI : http://dx.doi.org/10.13140/RG.2.2.24481.11366
Tkemaladze, J. (2025). Adaptive Systems and World Models. DOI : http://dx.doi.org/10.13140/RG.2.2.13617.90720
Tkemaladze, J. (2025). Allotransplantation Between Adult Drosophila of Different Ages and Sexes. DOI : http://dx.doi.org/10.13140/RG.2.2.27711.62884
Tkemaladze, J. (2025). Anti-Blastomic Substances in the Blood Plasma of Schizophrenia Patients. DOI : http://dx.doi.org/10.13140/RG.2.2.12721.08807
Tkemaladze, J. (2025). Centriole Elimination as a Mechanism for Restoring Cellular Order. DOI : http://dx.doi.org/10.13140/RG.2.2.12890.66248/1
Tkemaladze, J. (2025). Hypotheses on the Role of Centrioles in Aging Processes. DOI : http://dx.doi.org/10.13140/RG.2.2.15014.02887/1
Tkemaladze, J. (2025). Limits of Cellular Division: The Hayflick Phenomenon. DOI : http://dx.doi.org/10.13140/RG.2.2.25803.30249
Tkemaladze, J. (2025). Molecular Mechanisms of Aging and Modern Life Extension Strategies: From Antiquity to Mars Colonization. DOI : http://dx.doi.org/10.13140/RG.2.2.13208.51204
Tkemaladze, J. (2025). Pathways of Somatic Cell Specialization in Multicellular Organisms. DOI : http://dx.doi.org/10.13140/RG.2.2.23348.97929/1
Tkemaladze, J. (2025). Strategic Importance of the Caucasian Bridge and Global Power Rivalries. DOI : http://dx.doi.org/10.13140/RG.2.2.19153.03680
Tkemaladze, J. (2025). The Epistemological Reconfiguration and Transubstantial Reinterpretation of Eucharistic Practices Established by the Divine Figure of Jesus Christ in Relation to Theological Paradigms. DOI : http://dx.doi.org/10.13140/RG.2.2.28347.73769/1
Tkemaladze, J. (2025). Transforming the psyche with phoneme frequencies "Habere aliam linguam est possidere secundam animam". DOI : http://dx.doi.org/10.13140/RG.2.2.16105.61286
Tkemaladze, J. (2025). Uneven Centrosome Inheritance and Its Impact on Cell Fate. DOI : http://dx.doi.org/10.13140/RG.2.2.34917.31206
Tkemaladze, J. (2025). Ze World Model with Predicate Actualization and Filtering. DOI : http://dx.doi.org/10.13140/RG.2.2.15218.62407
Tkemaladze, J. (2025). Ze метод создания пластичного счетчика хронотропных частот чисел бесконечного потока информации. DOI : http://dx.doi.org/10.13140/RG.2.2.29162.43207
Tkemaladze, J. (2025). A Novel Integrated Bioprocessing Strategy for the Manufacturing of Shelf-Stable, Nutritionally Upgraded Activated Wheat: Development of a Comprehensive Protocol, In-Depth Nutritional Characterization, and Evaluation of Biofunctional Properties. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.16950787
Tkemaladze, J. (2025). Achieving Perpetual Vitality Through Innovation. DOI : http://dx.doi.org/10.13140/RG.2.2.31113.35685
Tkemaladze, J. (2025). Activated Wheat: The Power of Super Grains. Preprints. DOI : https://doi.org/10.20944/preprints202508.1724.v1
Tkemaladze, J. (2025). Adaptive Cognitive System Ze. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.15309162
Tkemaladze, J. (2025). Aging Model Based on Drosophila melanogaster: Mechanisms and Perspectives. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.14955643
Tkemaladze, J. (2025). Aging Model-Drosophila Melanogaster. DOI : http://dx.doi.org/10.13140/RG.2.2.16706.49607
Tkemaladze, J. (2025). An Interdisciplinary Study on the Causes of Antediluvian Longevity, the Postdiluvian Decline in Lifespan, and the Phenomenon of Job’s Life Extension. Preprints. DOI : https://doi.org/10.20944/preprints202509.1476.v1
Tkemaladze, J. (2025). Anatomy, Biogenesis, and Role in Cell Biology of Centrioles. Longevity Horizon, 1(2). DOI : https://doi.org/10.5281/zenodo.15051749
Tkemaladze, J. (2025). Anti-Blastomic Substances in the Plasma of Schizophrenia Patients: A Dual Role of Complement C4 in Synaptic Pruning and Tumor Suppression. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.15042448
Tkemaladze, J. (2025). Asymmetry in the Inheritance of Centrosomes/Centrioles and Its Consequences. Longevity Horizon, 1(2). DOI : https://doi.org/10.5281/zenodo.15053349
Tkemaladze, J. (2025). Bayesian Order in Ze. Longevity Horizon, 1(4). DOI : https://doi.org/10.5281/zenodo.17359987
Tkemaladze, J. (2025). Bayesian Priors Prediction in Ze. Longevity Horizon, 1(4). DOI : https://doi.org/10.5281/zenodo.17769150
Tkemaladze, J. (2025). Centriole Elimination: A Mechanism for Resetting Entropy in the Cell. Longevity Horizon, 1(2). DOI : https://doi.org/10.5281/zenodo.15053431
Tkemaladze, J. (2025). Concept of Death Awareness as an Existential Regulator in the Age of Biological Immortality. Longevity Horizon, 1(4). DOI : https://doi.org/10.5281/zenodo.17340207
Tkemaladze, J. (2025). Concept to The Alive Language. Longevity Horizon, 1(1). DOI : https://doi.org/10.5281/zenodo.14688792
Tkemaladze, J. (2025). Concept to The Caucasian Bridge. Longevity Horizon, 1(1). DOI : https://doi.org/10.5281/zenodo.17643013
Tkemaladze, J. (2025). Concept to The Curing All Diseases. Longevity Horizon, 1(1). DOI : https://doi.org/10.5281/zenodo.15048639
Tkemaladze, J. (2025). Concept to The Eternal Youth. Longevity Horizon, 1(1). DOI : https://doi.org/10.5281/zenodo.15048562
Tkemaladze, J. (2025). Concept to The Food Security. Longevity Horizon, 1(1). DOI : https://doi.org/10.5281/zenodo.15048716
Tkemaladze, J. (2025). Concept to the Living Space. Longevity Horizon, 1(1). DOI : https://doi.org/10.5281/zenodo.17208472
Tkemaladze, J. (2025). Concept to The Restoring Dogmas. Longevity Horizon, 1(1). DOI : https://doi.org/10.5281/zenodo.17175865
Tkemaladze, J. (2025). Differentiation of Somatic Cells in Multicellular Organisms. Longevity Horizon, 1(2). DOI : https://doi.org/10.5281/zenodo.15052436
Tkemaladze, J. (2025). Direct Reprogramming of Somatic Cells to Functional Gametes in Planarians via a Novel In Vitro Gametogenesis Protocol. Preprints. DOI : https://doi.org/10.20944/preprints202509.1071.v1
Tkemaladze, J. (2025). Heroic Self-Myth Hypothesis. Longevity Horizon, 1(4). DOI : https://doi.org/10.5281/zenodo.17711334
Tkemaladze, J. (2025). Induction of germline-like cells (PGCLCs). Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.16414775
Tkemaladze, J. (2025). Lake Aquaculture for Catastrophic Food Security. Longevity Horizon, 1(4). DOI : https://doi.org/10.5281/zenodo.17454164
Tkemaladze, J. (2025). Long-Lived Non-Renewable Structures in the Human Body. DOI : http://dx.doi.org/10.13140/RG.2.2.14826.43206
Tkemaladze, J. (2025). Mechanisms of Learning Through the Actualization of Discrepancies. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.15200612
Tkemaladze, J. (2025). Memorizing an Infinite Stream of Information in a Limited Memory Space: The Ze Method of a Plastic Counter of Chronotropic Number Frequencies. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.15170931
Tkemaladze, J. (2025). Molecular Insights and Radical Longevity from Ancient Elixirs to Mars Colonies. Longevity Horizon, 1(2). DOI : https://doi.org/10.5281/zenodo.15053947
Tkemaladze, J. (2025). Ontogenetic Permanence of Non-Renewable Biomechanical Configurations in Homo Sapiens Anatomy. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.15086387
Tkemaladze, J. (2025). Protocol for Transplantation of Healthy Cells Between Adult Drosophila of Different Ages and Sexes. Longevity Horizon, 1(2). DOI : https://doi.org/10.5281/zenodo.15053943
Tkemaladze, J. (2025). Replicative Hayflick Limit. Longevity Horizon, 1(2). DOI : https://doi.org/10.5281/zenodo.15052029
Tkemaladze, J. (2025). Solutions to the Living Space Problem to Overcome the Fear of Resurrection from the Dead. DOI : http://dx.doi.org/10.13140/RG.2.2.34655.57768
Tkemaladze, J. (2025). The Centriolar Theory of Differentiation Explains the Biological Meaning of the Centriolar Theory of Organismal Aging. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.15057288
Tkemaladze, J. (2025). The Centriolar Theory of Differentiation Explains the Biological Meaning of the.
Tkemaladze, J. (2025). The Concept of Data-Driven Automated Governance. Georgian Scientists, 6(4), 399–410. DOI : https://doi.org/10.52340/gs.2024.06.04.38
Tkemaladze, J. (2025). The Stage of Differentiation Into Mature Gametes During Gametogenesis in Vitro. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.16808827
Tkemaladze, J. (2025). The Tkemaladze Method Maps Cell Lineage with Mutant Mitochondrial Transfer. Longevity Horizon, 1(4). DOI : https://doi.org/10.5281/zenodo.17236869
Tkemaladze, J. (2025). The Tkemaladze Method: A Modernized Caucasian Technology for the Production of Shelf-Stable Activated Wheat with Enhanced Nutritional Properties. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.16905079
Tkemaladze, J. (2025). Theory of Lifespan Decline. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.17142909
Tkemaladze, J. (2025). Through In Vitro Gametogenesis — Young Stem Cells. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.15873113
Tkemaladze, J. (2025). Tkemaladze, J. (2025). The Centriole Paradox in Planarian Biology: Why Acentriolar Stem Cells Divide and Centriolar Somatic Cells Do Not. Preprints. DOI : https://doi.org/10.20944/preprints202509.0382.v1
Tkemaladze, J. (2025). Unlocking the Voynich Cipher via the New Algorithmic Coding Hypothesis. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.17054312
Tkemaladze, J. (2025). Uznadze Set Revisited. Longevity Horizon, 1(4). DOI : https://doi.org/10.5281/zenodo.17609772
Tkemaladze, J. (2025). Why do planarian cells without centrioles divide and cells with centrioles do not divide?. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.17054142
Tkemaladze, J. (2025). Гаметогенез In Vitro: современное состояние, технологии и перспективы применения. Research Gate. DOI : http://dx.doi.org/10.13140/RG.2.2.28647.36000
Tkemaladze, J. (2026). The Strength of Clay, The Weakness of Gods. Longevity Horizon, 2(1). DOI : https://doi.org/10.65649/2neyxv38
Tkemaladze, J. (2026). Visions of the Future. Longevity Horizon, 2(1). DOI : https://doi.org/10.65649/8be27s21
Tkemaladze, J. Systemic Resilience and Sustainable Nutritional Paradigms in Anthropogenic Ecosystems. DOI : http://dx.doi.org/10.13140/RG.2.2.18943.32169/1
Tkemaladze, J. V., & Chichinadze, K. N. (2005). Centriolar mechanisms of differentiation and replicative aging of higher animal cells. Biochemistry (Moscow), 70, 1288-1303.
Tkemaladze, J., & Apkhazava, D. (2019). Dasatinib and quercetin: short-term simultaneous administration improves physical capacity in human. J Biomedical Sci, 8(3), 3.
Tkemaladze, J., & Chichinadze, K. (2005). Potential role of centrioles in determining the morphogenetic status of animal somatic cells. Cell biology international, 29(5), 370-374.
Tkemaladze, J., & Chichinadze, K. (2010). Centriole, differentiation, and senescence. Rejuvenation research, 13(2-3), 339-342.
Tkemaladze, J., & Gakely, G. (2025). A Novel Biotechnological Approach for the Production of Shelf-Stable, Nutritionally Enhanced Activated Wheat: Protocol Development, Nutritional Profiling, and Bioactivity Assessment. DOI : https://doi.org/10.20944/preprints202508.1997.v1
Tkemaladze, J., & Gakely, G. (2025). Induction of de novo centriole biogenesis in planarian stem cells. Longevity Horizon, 1(4). DOI : https://doi.org/10.5281/zenodo.17283229
Tkemaladze, J., & Samanishvili, T. (2024). Mineral ice cream improves recovery of muscle functions after exercise. Georgian Scientists, 6(2), 36–50. DOI : https://doi.org/10.52340/gs.2024.06.02.04
Tkemaladze, J., Gakely, G., Gegelia, L., Papadopulo, I., Taktakidze, A., Metreveli, N., ... & Maglakelidze, U. (2025). Production of Functional Gametes from Somatic Cells of the Planarian Schmidtea Mediterranea Via in Vitro Gametogenesis. Longevity Horizon, 1(3). DOI : https://doi.org/10.5281/zenodo.17131291
Tkemaladze, J., Tavartkiladze, A., & Chichinadze, K. (2012). Programming and Implementation of Age-Related Changes. In Senescence. IntechOpen.
Tkemaladze, Jaba and Kipshidze, Mariam, Regeneration Potential of the Schmidtea Mediterranea CIW4 Planarian. Available at SSRN: https://ssrn.com/abstract=4633202 or http://dx.doi.org/10.2139/ssrn.4633202
Прангишвили, А. И., Гаситашвили, З. А., Мацаберидзе, М. И., Чичинадзе, К. Н., Ткемаладзе, Д. В., & Азмайпарашвили, З. А. (2017). К топологии антитеррористических и антикриминальных технологии для образовательных программ. В научном издании представлены материалы Десятой международной научно-технической конфе-ренции «Управление развитием крупномасштабных систем (MLSD’2016)» по следующим направле-ниям:• Проблемы управления развитием крупномасштабных систем, включая ТНК, Госхолдин-ги и Гос-корпорации., 284.
Прангишвили, А. И., Гаситашвили, З. А., Мацаберидзе, М. И., Чхартишвили, Л. С., Чичинадзе, К. Н., & Ткемаладзе, Д. В. (2017). & Азмайпарашвили, ЗА (2017). Системные составляющие здравоохранения и инноваций для организации европейской нано-биомедицинской екосистемной технологической платформы. Управление развитием крупномасштабных систем MLSD, 365-368.
Ткемаладзе, Д. (2025). Асимметрия в наследовании центросом/центриолей и ее последствия. DOI : http://dx.doi.org/110.13140/RG.2.2.34917.31206
Ткемаладзе, Д. (2025). Гаметогенез in vitro (IVG)-Этап дифференцировки в зрелые гаметы. DOI : http://dx.doi.org/10.13140/RG.2.2.20429.96482
Ткемаладзе, Д. (2025). Дифференциация соматических клеток многоклеточных животных. DOI : http://dx.doi.org/10.13140/RG.2.2.23348.97929/1
Ткемаладзе, Д. (2025). Индукция примордиальных клеток, подобных зародышевым клеткам (PGCLCs) современные достижения, механизмы и перспективы применения. DOI : http://dx.doi.org/10.13140/RG.2.2.27152.32004
Ткемаладзе, Д. (2025). Репликативный Лимит Хейфлика. DOI : http://dx.doi.org/10.13140/RG.2.2.25803.30249
Ткемаладзе, Д. (2025). Элиминация Центриолей: Механизм Обнуления Энтропии в Клетке. DOI : http://dx.doi.org/10.13140/RG.2.2.12890.66248/1
Ткемаладзе, Д. В., & Чичинадзе, К. Н. (2005). Центриолярные механизмы дифференцировки и репликативного старения клеток высших животных. Биохимия, 70(11), 1566-1584.
Ткемаладзе, Д., Цомаиа, Г., & Жоржолиани, И. (2001). Создание искусственных самоадаптирующихся систем на основе Теории Прогноза. Искусственный интеллект. УДК 004.89. Искусственный интеллект. УДК 004.89.
Чичинадзе, К. Н., & Ткемаладзе, Д. В. (2008). Центросомная гипотеза клеточного старения и дифференциации. Успехи геронтологии, 21(3), 367-371.
Чичинадзе, К., Ткемаладзе, Д., & Лазарашвили, А. (2012). Новый класс рнк и центросомная гипотеза старения клеток. Успехи геронтологии, 25(1), 23-28
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Laura Gegelia (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
